Recognition Sequence :
↑CATG
GTAC↓
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Reaction Temperature :
55°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; and 50% glycerol.
Ligation :
After 2-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Source :
An E.coli strain that carries the cloned Fat I gene from Flavobacterium aquatile NL3
Working buffer :
G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of DNA with 3 u.a. of enzyme for 16 hours at 55°C.
Concentration, u.a./ml :
5000
Inactivation :
20min Under 65°C
Methylation sensitivity :
Blocked by mCATG methylation
Reagents Supplied :
10 X SE-buffer G
Notes :
The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,25.
FatI cleaves linear plasmid DNA at a rate 1.5-2 times higher than supercoiled plasmid DNA.
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