Recognition Sequence :
↑(N)8AAGNNNNNCTT(N)13↑
↓(N)13TTCNNNNNGAA(N)8↓
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.
Ligation :
After 3-fold overdigestion with enzyme 20% of the DNA fragments can be ligated and of these 80% can be recut. In the presence of 10%PEG ligation is better.
Source :
Flavobacterium aquatile Ob10
Working buffer :
W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + SAM
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
1000-3000
Inactivation :
20min Under 65°C
Reagents Supplied :
10 X SE-buffer W, SAM
Notes :
High enzyme concentration results in star activity
To obtain 100% activity, SAM should be added to a final concentration 0.01 mM
Our Products Cannot Be Used As Medicines Directly For Personal Use.
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