Recognition Sequence :
CATG↑
↓GTAC
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol
Ligation :
After 3-fold overdigestion with enzyme more then 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut.
Source :
Flavobacterium aquatile N3
Working buffer :
SE-buffer FaeI (33 mM Tris-acetate (pH 8.3 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of pUC19 DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
500-2000
Inactivation :
20min Under 65°C
Reagents Supplied :
10 X SE-buffer FaeI, BSA
Notes :
To obtain 100% activity, BSA should be added the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Our Products Cannot Be Used As Medicines Directly For Personal Use.
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