Product Description :
E.coli Poly(A) Polymerase catalyze the addition of AMP converted from ATP to the 3' end of single-stranded RNA in a template-independent manner to form a Poly(A) tail. E. coli Poly(A) polymerase can use various single-stranded RNAs as substrate RNAs, but double-stranded RNAs and short oligonucleotides are not recommended as RNA substrates for this reaction, and DNA cannot be used as a substrate for this reaction substrate. The measured 15nt synthetic RNA can be used as a substrate for E. coli Poly(A) Polymerase, which catalyzes the formation of a Poly(A) tail in the presence of ATP. The Poly(A) tailing reaction catalyzed by E. coli Poly(A) Polymerase can only use ATP, not ADP or dATP. In addition, when CTP and UTP are used, their incorporation is less than 5% of that when ATP is used; and when GTP is used, it cannot be added to the 3' end of RNA at all.
Components :
·E. coli Poly(A) Polymerase (5U/µl)
·10X Reaction Buffer
Unit Definition :
One unit is defined as the amount of enzyme that will incorporate 1nmol of AMP into RNA in a 20µl volume in 10 minutes at 37 °C
Storage Buffer :
20mM Tris-HCl (pH7.5, 25°C), 500mM NaCl, 1mM DTT, 1mM EDTA, 50% (v/v) Glycerol, 0.1% (w/v) Triton X-100.
Size :
100U; 500U; 2.5KU; 10KU
Concentration, u.a./ml :
5U/µl
Inactivation :
Add EDTA to a final concentration of 10 mM, or heat at 65°C for 20 min.
Our Products Cannot Be Used As Medicines Directly For Personal Use.
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