Product Description :
E.coli Poly(A)Polymerase is a polymerase expressed by recombinant E.coli. The enzyme does not depend on the template and can catalyze the sequential incorporation of ATP into the 3' end of RNA in the form of AMP, that is, adding a polyadenosine tail to the 3' end of RNA. Poly(A) structure can improve the stability of RNA and improve the translation efficiency of mRNA in eukaryotic cells. Poly(A) polymerase has high tailing efficiency, capable of adding 20 - 200 A bases to the 3' end of RNA.
Components :
·E.coli Poly(A) Polymerase
Unit Definition :
The amount of enzyme required to incorporate 1 nmol of AMP into RNA in 10 minutes at 37°C and pH 8.0 is defined as 1 activity unit
Storage Buffer :
20 mM Tris-HCl (25℃, pH 7.5), 300 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% Glycerol
Concentration, u.a./ml :
5U/μl
Our Products Cannot Be Used As Medicines Directly For Personal Use.
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