Recognition Sequence :
CACNNN↑GTG
GTG↓NNNCAC
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol.
Ligation :
After 10-fold overdigestion with enzyme 70% of the DNA fragments can be ligated and recut. In the presence of 10%PEG ligation is better.
Source :
An E.coli strain, that carries the cloned gene Dra III from Deinococcus radiophilus
Working buffer :
2K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.) + BSA
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
5000
Inactivation :
20min Under 65°C
Reagents Supplied :
10 X SE-buffer 2K, BSA
Notes :
High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
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