DNase I

Cat :

GMP-005

Product Description :

DNase I, namely Deoxyribonuclease I (deoxyribonuclease I), is a deoxyribonuclease that can digest single-stranded or double-stranded DNA. This enzyme recognizes and cuts phosphodiester bonds, and the 5' end produced is a phosphate. group, a monodeoxynucleotide with a hydroxyl group at the 3' end or a single- or double-stranded oligodeoxynucleotide. The activity of DNase I depends on Ca2+ and can be activated by divalent metal ions Mg2+, Mn2+, etc. In the presence of Mg2+, this enzyme can randomly recognize and cut any site on any strand of double-stranded DNA; and in the presence of Mn2+, it can recognize and cut almost the same site on both strands of DNA, resulting in Blunt-ended or sticky-ended DNA fragments with a 1-2 nucleotide overhang.

Components :

·DNaseⅠ(1 U/μl)
·10X Reaction Buffer

Unit Definition :

The amount of enzyme required to completely degrade 1 μg of pUC19 plasmid DNA at 37°C for 10 min was defined as 1 activity unit (U).

Concentration, u.a./ml :

1U/μl

Storage :

Store at -20℃

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