Product Description :
DNase I is an endonuclease that digests single- or double-stranded DNA to produce single- or double-stranded oligodeoxynucleotides. The product of DNase I hydrolysis of single- or double-stranded DNA has a phosphate group at the 5' end and a hydroxyl group at the 3' end.
DNase I activity is dependent on calcium ions and can be activated by magnesium ions or divalent manganese ions. In the presence of magnesium ions, DNase I can randomly cleave any site of double-stranded DNA; in the presence of divalent manganese ions, DNase I can cleave DNA double-strands at the same site to form blunt ends, or 1-2 Sticky ends of nucleotide overhangs.
Components :
·DNase I, RNase-free(1U/μl)
·10X Reaction Buffer
·EDTA(25mM)
Unit Definition :
The amount of enzyme required to completely degrade 1 μg of pBR322 plasmid DNA within 10 minutes at 37°C was defined as 1 activity unit.
Storage Buffer :
50mM Tris-acetate(pH7.5), 10mM CaCl2, 50%(v/v)glycerol
Concentration, u.a./ml :
1U/μl
Inactivation :
DNase I was inactivated by heating at 65°C for 10 minutes after adding EDTA to a final concentration of 2.5mM. Phenol-chloroform extraction can also inactivate DNase I. Metal ion chelators, zinc ions at a concentration of mmol/L, 0.1% SDS, DTT, mercaptoethanol and other reducing agents, and salt concentrations above 50-100mM all have a significant inhibitory effect on DNase I.
Our Products Cannot Be Used As Medicines Directly For Personal Use.
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