Recognition Sequence :
GC↑GGCCGC
CGCCGG↓CG
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus-2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation :
After 5-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.
Source :
Curtobacterium citreus N
Assayed on :
Adenovirus-2 DNA
Working buffer :
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of DNA Ad-2 with 10 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
5000
Inactivation :
20min Under 65°C
Methylation sensitivity :
Blocked by CG methylation.
Reagents Supplied :
10 X SE-buffer Y.
Notes :
High enzyme concentration may result in star activity.
Our Products Cannot Be Used As Medicines Directly For Personal Use.
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