Recognition Sequence :
GAAGAC(N)2↑
CTTCTG(N)6↓
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Reaction Temperature :
55°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol.
Ligation :
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Source :
Bacillus stearothermophilus V2
Working buffer :
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 55°C.
Concentration, u.a./ml :
5000
Inactivation :
20min Under 65°C
Reagents Supplied :
10 X SE-buffer Y, BSA
Notes :
High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
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