Recognition Sequence :
GCAGC(N)8↑
CGTCG(N)12↓
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Reaction Temperature :
55°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol.
Ligation :
After 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Source :
Bacillus stearothermophilus V1
Working buffer :
G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 55°C.
Concentration, u.a./ml :
1000-2000
Inactivation :
20min Under 80°C
Reagents Supplied :
10 X SE-buffer G
Notes :
At 37°C activity is 10% from maximum.
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