Recognition Sequence :
C↑TRYAG
GAYRT↓C
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Reaction Temperature :
60°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol
Ligation :
After 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Source :
Bacillus stearothermophilus SF
Working buffer :
O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60°C.
Concentration, u.a./ml :
2000-5000
Inactivation :
20min Under 80°C
Reagents Supplied :
10 X SE-buffer O, BSA
Notes :
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
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