Recognition Sequence :
↑CCNGG
GGNCC↓
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 55°C in a total reaction volume of 50 μl.
Reaction Temperature :
55°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol.
Ligation :
After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Source :
Bacillus stearothermophilus SC
Assayed on :
Lambda DNA (dcm-)
Working buffer :
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 3 u.a. of enzyme for 16 hours at 55°C.
Concentration, u.a./ml :
2000-5000
Inactivation :
20min Under 80°C
Methylation sensitivity :
Blocked by Dcm-methylation (CmCWGG) CCWGG.
Reagents Supplied :
10 X SE-buffer Y
Notes :
At 37°C activity is 10% from maximum.
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