Recognition Sequence :
GACNN↑NNGTC
CTGNN↓NNCAG
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Reaction Temperature :
65°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol.
Ligation :
After 5-fold overdigestion with enzyme less than 5% of the DNA fragments can be ligated
Source :
Bacillus stearothermophillus PA
Working buffer :
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 25°C
Concentration, u.a./ml :
20000
Inactivation :
20min Under 80°C
Reagents Supplied :
10 X SE-buffer Y
Notes :
High enzyme concentration and incubation at 65°C for 16 hours results in star activity.
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