Recognition Sequence :
CGRY↑CG
GC↓YRGC
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl.
Reaction Temperature :
50°C
Storage Buffer :
10 mM Tris-HCl(pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.
Ligation :
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Source :
Bacillus stearothermophilus MC
Working buffer :
B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) + BSA
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 50°C.
Concentration, u.a./ml :
5000
Inactivation :
20min Under 80°C
Reagents Supplied :
10 X SE-buffer B, BSA
Notes :
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml
Do not use BSA for long incubation.
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