Recognition Sequence :
↑GATC
CTAG↓
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 65°C in a total reaction volume of 50 μl.
Reaction Temperature :
65°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA.
Ligation :
After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Source :
Bacillus stearothermophillus MB
Assayed on :
Lambda DNA (dam-)
Working buffer :
O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C.
Concentration, u.a./ml :
10000
Inactivation :
20min Under 80°C
Methylation sensitivity :
Blocked by overlapping Dam methylation(GmATC): GATC .
Not blocked by CG methylation.
Not cut hemimethylated site: 5`- G(6mA)TC-3` / 3`-CTAG-5`
Reagents Supplied :
10 X SE-buffer O
Our Products Cannot Be Used As Medicines Directly For Personal Use.
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