Recognition Sequence :
GCG↑C
C↓GCG
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl.
Reaction Temperature :
50°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol
Ligation :
After 40-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut
Source :
Bacillus stearothermophilus HH
Working buffer :
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of DNA with 100 u.a. of enzyme for 16 hours at 65°C.
Concentration, u.a./ml :
50000
Methylation sensitivity :
Blocked by
5`-G(5mC)GC-3`/3-CG(5mC)G-5` or
5`-G(5mC)GC-3`/3`-CGCG-5` methylation.
Not blocked by
5`-GCG(5mC)-3`/3`-(5mC)GCG-5` or
5`-GCG(5mC)-3`/3`-CGCG-5` methylation.
Reagents Supplied :
10 X SE-buffer Y, BSA
Notes :
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml
Do not use BSA for long incubation.
Our Products Cannot Be Used As Medicines Directly For Personal Use.
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