Recognition Sequence :
CCTNN↑NNNAGG
GGANNN↓NNTCC
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Reaction Temperature :
65°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.
Ligation :
After 5-fold overdigestion with enzyme about 60% of the DNA fragments can be ligated and recut. Of these 90% can be recut
Source :
Bacillus stearothermophillus EM
Working buffer :
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 65°C.
Concentration, u.a./ml :
2000-5000
Inactivation :
20min Under 80°C
Methylation sensitivity :
Not blocked by overlapping Dcm methylation(CmCWGG): CCTGGNNNAGG,
CCTNNNCCAGG.
Reagents Supplied :
10 X SE-buffer Y
Our Products Cannot Be Used As Medicines Directly For Personal Use.
PDF