Recognition Sequence :
ACN↑GT
TG↓NCA
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Reaction Temperature :
65°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.
Ligation :
After 10-fold overdigestion with enzyme ~50% of DNA fragments can be ligated.
Source :
Bacillus stearothermophilus 4C
Working buffer :
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Concentration, u.a./ml :
10000
Reagents Supplied :
10 X SE-buffer Y
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