Recognition Sequence :
GGTCTC(N)1↑
CCAGAG(N)5↓
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Reaction Temperature :
55°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol
Ligation :
After 5-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated. Of these 80% can be recut.
Source :
Bacillus stearothermophilus 31
Working buffer :
O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 10 u.a. of enzyme for 16 hours at 55°C.
Concentration, u.a./ml :
5000-10000
Inactivation :
20min Under 80°C
Methylation sensitivity :
Blocked by overlapping Dcm-methylation (CmCWGG): GAGACCWGG
Not blocked by methylation GGTCT(5mC)
Reagents Supplied :
10 X SE-buffer O, BSA
Notes :
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Our Products Cannot Be Used As Medicines Directly For Personal Use.
PDF