Recognition Sequence :
C↑GGCCG
GCCGG↓C
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 8.2); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.
Ligation :
After 5-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated. Of these 80% can be recut
Source :
Bacillus stearothermophilus X3
Working buffer :
O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 50°C.
Concentration, u.a./ml :
5000
Inactivation :
20min Under 80°C
Methylation sensitivity :
Blocked by CG methylation.
Reagents Supplied :
10 X SE-buffer O
Storage :
Store at -20°C. Storage at -70 °C is recommended for periods longer than 3 months
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