Bse3D I

Cat :

ET-1046RE

Recognition Sequence :

GCAATGNN↑
CGTTAC↓NN

Unit Definition :

One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.

Reaction Temperature :

60°C

Form :

Liquid

Storage Buffer :

10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol.

Ligation :

After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Source :

Bacillus stearothermophilus 3D

Assayed on :

Lambda DNA

Working buffer :

G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

Non-specific hydrolisis :

No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 60°C.

Size :

200U; 1000U

Concentration, u.a./ml :

5000

Inactivation :

20min Under 80°C

Reagents Supplied :

10 X SE-buffer G

Storage :

-20°C

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