Recognition Sequence :
CC↑TNAGC
GGANT↓CG
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol.
Ligation :
After 5-fold overdigestion with enzyme 80% of the DNA fragments can be ligated. Of these 90% can be recut. In the presence of 10% PEG ligation is better.
Source :
An E.coli strain, that carries recombinant plasmids
Working buffer :
K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM KCl; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 1 hours at 37°C.
Concentration, u.a./ml :
5000
Inactivation :
20min Under 65°C
Reagents Supplied :
10 X SE-buffer K
Notes :
High enzyme concentration may result in star activity or incomplete DNA cleavage. We recommend increasing incubation time instead of using an excess of Bpu10I.
The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,5.
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