Recognition Sequence :
ACTGGG(N)5↑
TGACCC(N)4↓
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol.
Ligation :
After 2-fold overdigestion with enzyme about 75% of the DNA fragments can be ligated and 95% may be recut.
Source :
Bacillus megaterium S87
Assayed on :
Lambda DNA (HindIII-digest)
Working buffer :
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Non-specific hydrolisis :
Overnight digest with BmuI is not recommended. A 50 μl reaction containing 1 μg of Lambda DNA and 0.5 units of enzyme incubated for 4 hours resulted in the same pattern of DNA bands as a reaction incubated for 1 hour.
Concentration, u.a./ml :
500-1000
Inactivation :
20min Under 65°C
Reagents Supplied :
10 X SE-buffer Y
Notes :
Enzyme is active in presence of EDTA.
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