Recognition Sequence :
G↑GATCC
CCTAG↓G
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 100 μg/ml BSA; 1 mM DTT; 50% glycerol.
Ligation :
After 50-fold overdigestion with enzyme approximately 90% of the DNA fragments can be ligated and recut.
Source :
An E.coli strain, that carries the cloned gene BamHI from Bacillus amyloliquefaciens H
Working buffer :
G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Size :
4000U; 20000U; 4000U; 20000U;
Concentration, u.a./ml :
20000; 50000
Inactivation :
20min Under 65°C
Methylation sensitivity :
Not blocked by Dam methylation (GmATC) GGATCC
Reagents Supplied :
10 X SE-buffer G, BSA
Notes :
High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Our Products Cannot Be Used As Medicines Directly For Personal Use.
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