Recognition Sequence :
GCG↑C
C↓GCG
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol.
Ligation :
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Source :
Arthrobacter species LE3860
Working buffer :
O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
10000
Inactivation :
20min Under 65°C
Reagents Supplied :
10 X SE-buffer O
Notes :
Blocked by
5'-G(5mC)GC-3'/3-CG(5mC)G-5' methylation.
Not blocked by
5'-GCG(5mC)-3'/3`-(5mC)GCG-5` or
5'-GCG(5mC)-3'/3'-CGCG-5' methylation.
Cut hemimethylated site: 5'-G(5mC)GC-3'/3'-CGCG-5'
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