Recognition Sequence :
↑(N)8GACNNNNNNTTYG(N)11↑
↓(N)13CTGNNNNNNAARC(N)6↓
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 30°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Storage Buffer :
10 mM KH2PO4(pH 7.4); 200 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol.
Ligation :
After 3-fold overdigestion with enzyme 70% of DNA fragments can be ligated. Of these 80% can be recut.
Source :
Arthrobacter species NTS
Working buffer :
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 1 u.a. of enzyme for 16 hours at 30°C.
Concentration, u.a./ml :
3000-5000
Inactivation :
20min Under 65°C
Reagents Supplied :
10 X SE-buffer Y, BSA
Notes :
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Our Products Cannot Be Used As Medicines Directly For Personal Use.
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