Acl I | Creative Enzymes

Acl I

Cat :

ET-1009RE

Recognition Sequence :

AA↑CGTT
TTGC↓AA

Unit Definition :

One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Reaction Temperature :

37°C

Form :

Liquid

Storage Buffer :

10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 0.05% Triton X-100; 50% glycerol

Ligation :

After 2-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.

Source :

Acinetobacter calcoaceticus

Assayed on :

Lambda DNA

Working buffer :

Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Non-specific hydrolisis :

No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C.

Size :

200U

Concentration, u.a./ml :

3000-5000

Inactivation :

20min Under 65°C

Methylation sensitivity :

Blocked by CG methylation

Reagents Supplied :

10 X SE-buffer Y, BSA

Storage :

-20°C

Notes :

To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.

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