AccBS I

Cat :
ET-1008RE
Recognition Sequence :
GAG↑CGG
CTC↓GCC
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Form :
Liquid
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol
Ligation :
After 5-fold overdigestion with enzyme 90% of DNA fragments can be ligated. Of these 50% can be recut.
Source :
Acinetobacter calcoaceticus BS
Assayed on :
Lambda DNA
Working buffer :
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
AccBS I
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Size :
1000U; 5000U
Concentration, u.a./ml :
10000
Inactivation :
20min Under 65°C
Reagents Supplied :
10 X SE-buffer Y
Storage :
-20°C

Inquiry

We are here to answer any question you may have