Acc65 I

Cat :
ET-1005RE
Recognition Sequence :
G↑GTACC
CCATG↓G
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA(dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Form :
Liquid
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol
Ligation :
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Source :
Acinetobacter calcoaceticus 65
Assayed on :
Lambda DNA(dcm-)
Working buffer :
W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Acc65 I
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Size :
1000U; 5000U
Concentration, u.a./ml :
20000
Inactivation :
20min Under 65°C
Methylation sensitivity :
Blocked by overlapping Dcm methylation(CmCWGG): GGTACCWGG.
Reagents Supplied :
10 X SE-buffer W
Storage :
-20°C

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