Acc16 I

Cat :
ET-1003RE
Recognition Sequence :
TGC↑GCA
ACG↓CGT
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
37°C
Form :
Liquid
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol
Ligation :
After 5-fold overdigestion with enzyme about >80% of the DNA fragments can be ligated and recut.
Source :
Acinetobacter calcoaceticus 16
Assayed on :
Lambda DNA
Working buffer :
W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Acc16 I
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Size :
200U; 1000U
Concentration, u.a./ml :
5000
Inactivation :
20min Under 65°C
Reagents Supplied :
10 X SE-buffer W
Storage :
-20°C

Inquiry

We are here to answer any question you may have