Recognition Sequence :
Unit Definition :
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction Temperature :
Storage Buffer :
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 200 μg/ml BSA; 1 mM DTT; and 50% glycerol.
After 10-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut.
An E.coli strain, that carries the cloned Aat II gene from Acetobacter aceti
Working buffer :
SE-Buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Non-specific hydrolisis :
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Concentration, u.a./ml :
20min Under 65°C
Reagents Supplied :
10X SE-buffer Y
High enzyme concentration may result in star activity.